Abstract: AMP activated protein kinase (AMPK) is a kind of kinase that widely involved in various metabolic regulation,which is the main cellular energy sensor.AMPK is activated by the Thr172 phosphorylation of alpha subunit,which can control lipid synthesis and glucose stability by the inhibition of the related transcription factor in lipid synthesis and hepatic gluconeogenesis.Liver gluconeogenesis is regulated by the hormone and crosstalks among transcription factors such as FOXO1,CREB,TORC2,HNF-4α,PGC-1α and eventually put the signal back to the two key enzyme genes PEPCK and G6Pase of gluconeogenesis.The activated AMPK can inhibit expression of key gluconeogenic enzymes and transcription factors,thereby inhibition of hepatic gluconeogenesis and reducing the blood glucose level.In this review,we aim to discuss and summarize the role of AMPK in liver glucolipid metabolism,focusing on the analysis of the link between AMPK and transcription factors of liver gluconeogenesis signaling.