高级检索

    灵芝孢子油指纹图谱研究及其混淆品鉴别

    Studies on Fingerprint of Oil of Ganoderma Lucidum Spore and Identification for It's Confusion

      摘要
    • 摘要: 目的 建立灵芝孢子油HPLC指纹图谱并鉴别其油类混淆品。方法 采用HPLC，色谱柱为ACCHROMUnitary-C18（250 mm×4.6 mm，5 μm），流动相为乙腈-异丙醇（51：49），流速为1.0 mL·min-1，柱温为30℃，以蒸发光散射检测。结果 建立了灵芝孢子油脂肪酸酯类成分HPLC指纹图谱，确定了8个共有峰，分别为三亚油酸甘油酯、1，2-二亚油酸-3-油酸甘油酯、1，2-二亚油酸-3-棕榈酸甘油酯、1，2-二油酸-3-亚油酸甘油酯、1-棕榈酸-2-油酸-3-亚油酸甘油酯、三油酸甘油酯、1，2-二油酸-3-棕榈酸甘油酯、1，2-二油酸-3-硬脂酸甘油酯。灵芝孢子油混淆品的图谱与灵芝孢子油指纹图谱有明显区别。结论 建立的灵芝孢子油脂肪酸酯类成分指纹图谱能很好地区别灵芝孢子油和其混淆品。

       

      Abstract: OBJECTIVE To establish a specific HPLC fingerprint of the oil of Ganoderma lucidum spore and apply for identifying for its confusion.METHODS The HPLC chromatography was performed on an ACCHROM Unitary-C18 column (250 mm×4.6 mm,5 μm) with the mobile phase of acetonitrile-isopropanol (51︰49).The flow rate was 1.0 mL·min-1 at 30℃ with the detection of ELSD.RESULTS The chromatographic fingerprint of aliphatic esters of oil of Ganoderma lucidum spore was established.The fingerprint contained 8 recognizable peaks of trilinolein,1,2-linolein-3-olein,1,2-linolein-3-palmitin,1,2-olein-3-linolein,1-palmitin-2-olein-3-linolein,triolein,1,2-olein-3-palmitin and 1,2-olein-3-stearin.The fingerprint of oil of Ganoderma lucidum spore was different from its confution obviously.CONCLUSION The established fingerprint can distinguish oil of Ganoderma lucidum spore from its confusion.

       

      • HTML全文
      • 参考文献(11)
      • 相关文章
      • 施引文献
    • 资源附件(0)

    /

    返回文章
    返回