Abstract: OBJECTIVE To detect the acute toxicity of Cichorium glandulosum Boiss.et Huet aqueous extracts (MS),and to research the protective effect of MS on acute liver injury mice induced by D-GaIN,BCG+LPS and ANIT.METHODS The mice were randomly divided into the treatment group and the normal group in acute toxicity test.The mortality,changes of the body weigh and feed intake of animals were measured.After 14 d,all mice were killed and grossly anatomized,then the internal organs were observed.Liver-protective experiment:Mice were divided into normal group,model group,positive drug group,the low and high dose groups of MS (0.13,0.26 g·kg^-1).Acute liver injury mice mode1 were induced by D-GaIN,BCG+LPS and ANIT.Then blood from eyeball was used to examine level of serum ALT,AST,TBIL and DBIL.The 1iver index was determined.And the pathologic section of the 1iver was stained for HE expression.RESULTS Acute toxicity test in mice to the single dose of maximum dose of 150 g·kg^-1 without death and other acute toxicity reaction.In D-GaIN induced chemical liver injury model,MS significantly reduced D-GaIN induced ALT,AST and TBIL at 0.26 g·kg^-1(P<0.05 or P<0.01).In the immune liver injury mode,MS significantly (P<0.01) reduced BCG+LPS induced ALT,AST at 0.13 and 0.26 g·kg^-1,but had no influence on TBIL.In the model of jaundice induced liver injury,MS significantly reduced ANIT induced ALT,AST,TBIL and DBIL at 0.26 g·kg^-1(P<0.01).In addition,MS significantly reduced TBIL and DBIL at 0.13 g·kg^-1(P<0.01).Pathologic examination showed liver injury had significantly improved in each treatment groups.CONCLUSION There are not exist the acute toxicity of MS in treatment mice with maximum dose 150 g·kg^-1.And,MS has a protective effect on acute liver injury.