Abstract: OBJECTIVE To observe the effect of Qingliang ointment on the expression of vascular endothelial growth factor (VEGF),angiopoientin-2(ANG-2),CD34 and hydroxyproline content in granulation tissue of incisional wound after anorectal operation.METHODS Fourty-two cases of anorectal surgery patients were randomly divided into control group and Qingliang ointment group,the control group of patients with external application of Vaseline gauze wound,while the Qingliang ointment group with external application of Qingliang ointment.The expression of VEGF,ANG-2,CD34 and hydroxyproline content were detected on the 3rd day,7th day and 14th day after operation.RESULTS Compared with the 3rd day,the expression of VEGF,ANG-2 and CD34 and the content of hydroxyproline in control group and Qingliang ointment group were gradually increased (P<0.05) on the 7th day and the 14th day after operation in the granulation tissue.The expression of VEGF,ANG-2,CD34 protein and hydroxyproline in the wound granulation tissue of Qinglian ointment group and control group had no significant difference on the 3rd and 7th day after operation.The expression of VEGF,ANG-2,CD34 and the content of hydroxyproline in granulation tissues of wound group were increased (P<0.05) on the 14th day after operation compared with the control group.CONCLUSION Qingliang ointment can promote the wound healing by promoting the expression of VEGF,ANG-2,CD34 protein and the increase of hydroxyproline content.