Abstract: OBJECTIVE To established a quantitative analysis of multi-components with a single marker (QAMS) method for simultaneous quantitative analysis of the five flavonoids in Oroxyli Semen based on full-time multi-wavelength fusion.METHODS With UPLC methods,Eclipse Plus C₁₈ column (2.1 mm×100 mm,1.8 μm) was used,the mobile phase was 0.1% methanoic acid in water (A) and acetonitrile (B) with gradient elution (0-10 min,15%→57% B;10-15 min,57%→65% B).The detection wavelengths were 256,280,300,320 and 350 nm.The flow rate of the mobile phase was 0.4 mL·min^-1 and the temperature was 30 ℃. RESULTS The ruggedness of relative correction factor was assayed and data obtained from the two methods (ESM and QAMS) were compared,and no statistically significant difference was observed,which indicated that the new approached was applicable in the quantitative analysis of Oroxyli Semen.CONCLUSION A new method is established for simultaneous quantitative determination of five bioactive flavonoids from traditional Chinese medicine Oroxyli Semen by combining full-time multi-wavelength fusion with QAMS,through applicability experiment.The method is simple,accurate and reliable,which is suitable for the quality evaluation of Oroxyli Semen.