Abstract: OBJECTIVE To research the characteristics of Ganoderma lucidum spore oil triglycerides was investigated by HPLC-ELSD. METHODS The chromatographic separation was performed on an ACCHROM Unitary-C₁₈ column (4.6 mm×250 mm, 5 μm) with the mobile phase of acetonitrile-isopropanol(51:49). The flow rate was 1.0 mL·min^-1 at 30℃ with the detection by ELSD. RESULTS The calibration curve was linear for trilinolein, 1,2-linolein-3-olein, 1,2-linolein-3-palmitin, 1,2-olein-3-linolein, 1-palmitin-2-olein-3-linolein, triolein, 1,2-olein-3-palmitin and 1, 2-olein-3-stearin in the range of 0.028 70-0.861 0 µg, 0.146 8-4.404 µg, 0.050 75-1.523 µg, 0.279 6-8.388 µg, 0.242 1-7.263 µg, 0.613 2^-18.40 µg, 0.341 6^-10.25 µg, 0.069 15-2.075 µg (r²=0.999 3, 0.998 8, 0.999 4, 0.999 3, 0.998 9, 0.998 9, 0.999 0 and 0.999 5), respectively. The average recovery was 103.3%, 99.0%, 103.3%, 97.9%, 100.1%, 104.4%, 96.4% and 105.0% with the RSD were 4.9%, 4.7%, 3.8%, 5.0%, 5.0%, 3.4%, 4.6% and 3.2%, respectively. The main components were 1,2-olein-3-palmitin, triolein, 1,2-olein-3-linolein, 1-palmitin-2-olein-3-linolein, 1, 2-linolein-3-olein, accounted for 90% of the total. CONCLUSION The method is rapid, simple and shows good specificity and reproducibility. It can be used for quality control and adulteration analysis of Ganoderma lucidum spore oil.