Abstract: OBJECTIVE To provide a rapid and simple method for identifying Stachyuri Medulla and its adulterants, based on Near-Infrared spectroscopy. METHODS The conformity test mode and cluster analysis mode were established after pre-treatment by the second derivative combining vector normalization method, in which the conformity index limit was set as 3.6 and the smooth points were 13. RESULTS The conformity test and cluster analysis results based on the wavelength range of 6 202.2-5 199.4 cm^-1 and 4 701.8-4 196.5 cm^-1, were coincident with variety identification. The Near-Infrared spectroscopy method could identify stachyuri medulla and its adulterants. CONCLUSION This method can be used for the rapid and accurate diferentiation of them, and may be meaningful for the research on the quality analysis of stachyuri medulla.