Abstract: OBJECTIVE To evaluate eye irritation potential of different shampoos using Draize assay in vivo and eye irritation test with human cornea-like epithelial tissue model BioOcular^TM in vitro respectively. METHODS In terms of OECD Guide Line 492, 8 kinds of different shampoos were tested both in Draize assay in vivo and eye irritation test with reconstructed human cornea-like epithelial tissue model BioOcular^TM in vitro. RESULTS There was a good relativity of eye irritation potential for these two methods. And correlation coefficent score for these two methods was 0.955. CONCLUSION Compared with Draize assay in vivo human cornea-like epithelial tissue model BioOcular^TM in vitro needs short period, simple steps, and the results are relatively objective. It is workable to use 3D human cornea-like epithelial tissue model BioOcular^TM in vitro for eye irritation test partly instead of Draize assay.