Abstract: OBJECTIVE To establish and improve the quality standard of Ruangan Suopi pill. METHODS Microscopic identification and TLC were conducted for the qualitative identification of Astragali radix, Salviae miltiorrhizae radix et rhizoma, Paeoniae radix rubra, Trionycis carapax, Rhei radix et rhizoma, Schisandrae chinensis fructus, Persicae semen, Notoginseng radix et rhizoma in Ruangan Suopi pill; HPLC was performed to determine the contents of paeoniflorin and salvianolic acid B. It was performed on column of Wondasil C₁₈(250 mm×4.6 mm, 5 μm) with mobile phase of acetonitrile -0.1% phosphonic acid in linear gradient elution mode at the flow rate of 1.0 mL·min^-1, the UV detection wavelength of 230 nm and 286 nm, the column temperature of 30 ℃ and the volume of 10 μL. RESULTS The microscopic identification showed microscopic characteristics of four traditional Chinese medicines. TLC spots of the five traditional Chinese medicines were clear and well-separated without negative interference. The HPLC experiment results showed the good linearities in the range of 9.79-195.80 μg·mL^-1 (r=0.999 9) for paeoniflorin, 11.45-229.00 μg·mL^-1 (r=0.999 9) for salvianolic acid B, respectively. The average recovery rates of two compounds were 100.6%, 101.9%, and RSD were 1.26%, 1.05%. CONCLUSION The method is rapid, convenient, accurate, repeatable, and suitable for quality control of Ruangan Suopi pill.