Abstract: OBJECTIVE To develop an HPLC method for determination of tannin-gallic acid and ellagic acid in traditional SHE medicine Rubi Radix et Rhizoma, and to confirm its best harvest time. METHODS The HPLC was applied with the chromatographic condition as follows:the chromatographic column was Agilent Zorbax SB-C₁₈(4.6 mm×250 mm, 5 μm) with column temperature of 30℃; the mobile phase was a mixed liquid with methanol-0.1% H₃PO₄ solution (gradient elution) at a flow rate of 1.0 mL·min^-1; and the detection wavelength was 263 nm. RESULTS There was no significant difference of the content of gallic acid between different months, while the content of ellagic acid was significantly different, and both content was the highest in November to January. CONCLUSION It is suggested that the best harvest time of Rubi Radix et Rhizoma of tannin (gallic acid and ellagic acid) is in November to January.