Abstract: OBJECTIVE To optimize ultrasonic-assisted extraction process of total alkaloids from Corydalis conspersa Maxim. METHODS the hydrochloric acid concentration, liquid-feed ratio and extraction temperature, which were major factors influencing the extraction according to the single factor experiments, were selected as independent variables, and through using the extraction rate of total alkaloids as response values and making the Box-Benhken central combinational design, the effects of the above factors on the extraction were estimated by response surface methodology. RESULTS The results indicated that the optimal extraction conditions were as follows:35 mL·g^-1 of liquid-feed ratio, 0.4% of hydrochloric acid concentration, and 75℃ extraction temperature. Under these conditions, the content of total alkaloids was 0.46 mg·g^-1 from Corydalis conspersa Maxim by ultraviolet spectrophotometer. CONCLUSION The optimized parameters from the expriments are accurate, which can be used for the extraction and production of total alkaloids from Corydalis conspersa Maxim.