Abstract: OBJECTIVE To investigate the correlation between genotyping of breast cancer and blood serum tumor markers. METHODS The serum tumors markers were examined by electrochemistry illumination and radioimmunoassay in 106 patients with breast cancer from January 2013 to December 2015, ER, PR and c-erbB-2 were detected by immunohistochemistry. Tumor markers were detected by electrochemiluminescence, one-way ANOVA was used to decide significant differences in categories. RESULTS Increase of serum CYFRA21-1, FER, CEA, CA724 level was correlated with Luminal A, significantly higher than the other 3 genotyping, the difference was statistically significant(P<0.05). CA125, CA153 level was correlated with Luminal A and Basal-like, significantly higher than the other 2 genotyping, the difference was statistically significant (P<0.05). There were no significant differences in other tumor markers in different genotypes. CONCLUSION CYFRA21-1, FER, CEA, CA724, CA125, CA153 level in breast cancer have correlation with genotyping.