Abstract: OBJECTIVE An HPLC-ELSD method was applied to determine the content changes of lysophosphatidylcholine (LPC) in fat emulsion injections. METHODS A propylene glycol bonded silica gel column was used, with the mobile phase A of hexane:isopropyl alcohol (40:60) and B of isopropyl alcohol:water (80:20) by gradient elution and with the application of ELSD. RESULTS The fat emulsion injections were unstable at high temperatures and it should be stored at about 25℃. CONCLUSION The limited contents of LPC in fat emulsion injections could meet the related drug standard both in the accelerated test and long-term test.