Abstract: OBJECTIVE To prepare donepezil ethosomes, and further optimize the transdermal delivery of drugs. METHODS Infusing method was used to prepare donepezil ethosomes. The systems were characterized for shape, particle size and entrapment efficiency (EE). Franz diffusion cells and confocal laser scanning microscopy were used for the percutaneous absorption studies. RESULTS The EE of donepezil ethosomes(ethanol content 45%) were much higher than that of liposome. The percentage of the drug in ethosomes retained by isolated skin were 3 and 1.6 times higher than liposomes and hydroalcoholic solution. CONCLUSION Ethosomes may be able to enhance the permeation of donepezil into skin.