Abstract: OBJECTIVE To evaluate the influence of the storage year and places of origin on the quality of medicinal materials through the quality analysis for the Croci Stigma of different years and different places. METHODS The Croci Stigma of different years and different places were collected to study the quality inspection, mainly including the identification and content determination of medicinal herbs. RESULTS As the extension of storage year, the Croci Stigma had faded in different degrees, and the total glycosides content of Croci Stigma showed a trend of decrease; Compared the Croci Stigma of different regions, the results showed that the total glycosides content of Croci Stigma made in Shanghai and Jiande were >10%, some batches of Croci Stigma abroad didn't reach the requirements of pharmacopoeia. CONCLUSION As the extension of storage year, the appearance of the Croci Stigma and the content of total glycosides in Croci Stigma will be a big change, while taking some special preservation measures can inhibit the decrease of its content; and the Croci Stigma glycosides content in different places are also different, domestic medicinal materials have higher glycosidescontent of Croci Stigma, which may be related to the processing and preservation methods.