Abstract: OBJECTIVE To investigate the antioxidant effects of water extract from leaves of Lonicera hypoglauca Miq. and provide a scientific basis for the development and utilization of the leaves. METHODS Study the antioxidant activities of the different ethanol sediments from water extract by the experiments in vivo and in vitro. Compared with Vc and BHT, the in vitro antioxidant activities were studied by DPPH radical scavenging and the reducing capacity of Fe³⁺. Senile mouse models induced by D-galactose were used. Compared with Vc, the in vivo antioxidant activities of sediments were studied by measuring SOD, GSH-PX activity and MDA content of serum and liver tissue. RESULTS Experiments in vitro (0.1-5 mg·mL^-1):DPPH radical scavenging experiments:the 90% and the 30% ethanol precipitation and the filtrates were better than the others. The reducing capacity of Fe³⁺:the 90% and the 30% ethanol precipitation and the filtrates had better effect than the other various precipitations. Experiments in vivo, the comprehensive results of SOD:the 30% precipitation(high dose group) and the 90% precipitation(low dose group) had the more excellent performance than Vc. Comprehensive GSH-PX activity results:the 30% precipitation(high dose group), the 90% precipitation(high dose group) and the 90% filtrate (low dose group) had good activities than the other groups. Comprehensive MDA activity results:30% precipitation(both the high and low dose group), 30% filtrate(high dose group) and 90% precipitation (low dose group) were better than the others. CONCLUSION The various eparation components all have good antioxidant activities both in vitro and in vivo. Considering the indicators both in vitro and in vivo, the 30% achol precipitation is the best and it's antioxidant activity is worthy of further research.