Abstract: OBJECTIVE To study the lipid-lowering efficacy in different components of grape seed proanthocyanidins. METHODS Different concentrations of ethanol percolation extraction, macroporous resin separation and purification were used to obtain 10%, 30%, 50%, 70% ethanol portion of grape seed proanthocyanidin extract. Rats hyperlipidemia model was established, and the content of glycerol triester (TG), total cholesterol (TC), alanine aminotransferase (ALT), aspartate aminotransferase (AST) in each groups' serum were determined, Lee'S index and organ index were calculated as well. According to each extract pharmacodynamic study to determine the grape seed proanthocyanidins lipid-lowering effective parts. RESULTS Grape seed proanthocyanidin extract 10%, 30%, 50% ethanol parts could significantly reduce high cholesterol TG content in hyperlipidemia model rats(P<0.01); while 50% ethanol part could also significantly reduce the hyperlipidemia ALT and AST content in model rats (P<0.01); Compared with the model group, the resulting group elution kidney index increased significantly after administration of 10% ethanol (P<0.01); 10%, 30%, 50%, 70% ethanol eluted income group significantly increased spleen index (P<0.05); eluted with 30% ethanol resulting group can significantly reduce the total lipid index (P<0.05); 10%, 50%, 70% ethanol eluted income group can significantly reduce the total lipid index (P<0.01). CONCLUSION The 50% ethanol part of grape seed proanthocyanidins play an effective affect in lipid-lowering efficacy of grape seed extract.