ASE-HPLC测定保和丸中橙皮苷的含量

Determination of Hesperidin in Bohol Pill by ASE-HPLC

摘要: 目的建立加速溶剂萃取（accelerated solvent extraction，ASE）-高效液相色谱法测定保和丸中橙皮苷含量的方法。方法利用ASE提取保和丸中橙皮苷，采用HPLC测定保和丸中橙皮苷的含量，选用Kinete×2.6μXB-C₁₈ 100A色谱柱（100 mm×4.6mm，5 μm），流动相为甲醇-7%醋酸溶液（37：63），流速0.7 mL·min^-1，柱温40℃，检测波长283 nm。结果橙皮苷在3.707~74.140 μg·mL^-1内与峰面积呈良好线性关系，平均加样回收率为99.33%，RSD为0.2%。结论该方法快速、准确，可用于保和丸中橙皮苷含量的测定。

Abstract: OBJECTIVE To develop a method for the determination of hesperidin in Bohol pill by accelerated solvent extraction(ASE)-HPLC method. METHODS Hesperidin in Bohol pill was extracted by ASE. The analytical column was Kinetex XB-C₁₈(100 mm×4.60 mm, 2.6 mm). The mobile phase was methanol-7% acetic acid(37:63) with flow rate 0.7 mL·min^-1. The column temperature was 40℃. The detector wavelength was set at 283 nm. RESULTS The linear range was 3.707-74.140 μg·mL^-1. The average recovery of hesperidin was 99.33%(RSD=0.2%). CONCLUSION The established method is simple, rapid and reliable, which can be applied to determine hesperidin in Bohol pill.