Abstract: OBJECTIVE To develop an HPLC with wavelength switching combined gradient elution method for simultaneous determination of caffeic acid, rosmarinic acid, 3'-hydroxy puerarin, puerarin, 3'-methoxypuerarin and daidzin in Shensu Xuanfei Wan. METHODS ZOBAX SB-C₁₈ column (4.6 mm×250 mm,5 μm) was applied with methanol-acetonitrile (1:1) (A) and 0.1% glacial acetic acid solution(B) as mobile phase in gradient mode. The flow rate was 1.0 mL·min^-1. Caffeic acid and rosmarinic acid were detected at 320 nm, 3'-hydroxy puerarin, puerarin, 3'-methoxypuerarin and daidzin were detected at 250 nm. Injection volume was 20 μL. RESULTS The concentration and peak area of caffeic acid, rosmarinic acid, 3'-hydroxy puerarin, puerarin, 3'-methoxypuerarin and daidzin were at 2.32~46.40 μg·mL^-1(r=0.999 8), 3.06~61.20 μg·mL^-1 (r=0.999 5), 4.45~89.00 μg·mL^-1(r=0.999 9), 14.48~289.60 μg·mL^-1(r=0.999 9), 4.86~97.20 μg·mL^-1(r=0.999 7), 3.69~73.80 μg·mL^-1 (r=0.999 6), respectively, and showed a good linear relationship. The average recoveries and the corresponding RSD were 99.0%(1.6%), 97.5% (0.6%), 99.3%(0.9%), 98.6%(1.3%), 97.6%(1.3%), 97.0%(0.9%), respectively. CONCLUSION The method is accurate, quick, simple. It could be reliable for the quality control of Shensu Xuanfei Wan.