Abstract: OBJECTIVE To optimize the preparation technology of β-elemene-loaded nano polymeric micelles. METHODS With poly(ethylene glycol-2000)-grafted distearoy lphosphosphatidylethanolamine(DSPE-PEG2000) as the carrier materials, thin film hydration method was used to prepare β-elemene-loaded nano polymeric micelles; With entrapment efficiency of β-elemene as index, orthogonal design was adopted to optimize effects of the ratio of β-elemene and DSPE-PEG2000, volumn and time of hydration, pH of PBS. RESULTS In the optimal conditions, the mass ratio of β-elemene and DSPE-PEG2000 of 1:10, hydration volume of 5 mL, hydration time of 30 min, PBS buffer pH of 7.4, the prepared nanoparticles were round and regular in shape without adhesions, the average of particle size was 56 nm, the average of entrapment efficiency was 88.83%. CONCLUSION The preparation established in this optimization method is stable and feasible, and it could be used for the preparation of β-elemene-loaded nano polymeric micelles.