Abstract: OBJECTIVE To establish the HPLC fingerprints of chemical constituents in Cremastrae Pseudobulbus Pleiones Pseudobulbus and provide the basis for the quality evaluate of it. METHODS The chromatographic separation was performed on an Agilent 1200 Topsil^TM C₁₈(4.6 mm×250 mm, 5 μm) column, and the mobile phase was consisted of acetonitrile and water with gradient elution. The flow rate was 1.0 mL·min^-1, the detection wavelength was 224 nm, and the column temperature was 30℃. RESULTS There were 7 common peaks in the HPLC fingerprints of 10 batches of Cremastrae Pseudobulbus Pleiones Pseudobulbus, and the 7 characteristic peaks showed obvious difference in Cremastrae Pseudobulbus Pleiones Pseudobulbus from different habitats. Significant difference was observed in the militarine content among the samples. CONCLUSION The HPLC fingerprints method is simple, rapid and feasible in the quality control of Cremastrae Pseudobulbus Pleiones Pseudobulbus.