Abstract: OBJECTIVE To investigate the role and mechanism of resveratrol in cisplatin-induced cell death in bladder cancer. METHODS MTT assay as well as flow cytometry was performed to detect the cell death and apoptosis respectively in bladder cancer cell line T24 treated with resveratrol and cisplatin. The uptake of glucose and the production of lactate and ATP were evaluated after the T24 cells were treated with resveratrol and cisplatin. Western blot analysis was performed to detect the expression of PKM2 in T24 cells treated with resveratrol and cisplatin. PKM2 eukaryotic expression vector was conducted to investigate its role in combination treatment with resveratrol and cisplatin in T24. RESULTS Although resveratrol exhibited low anti-tumor effect on T24 cells, it significantly enhanced the cell death induced by cisplatin. Resveratrol significantly impaired the glucose uptake and the production of lactate and ATP in T24 cells. Furthermore, the expression of PKM2 was down-regulated due to the resveratrol treatment. Finally, we found the transfection of PKM2 eukaryotic expression vector abolished the synergistic effect of resveratrol on cisplatin-induced cell death in T24 cells. CONCLUSION Resveratrol promotes cisplatin-induced cell death by inhibiting the glucose metabolism via PKM2 pathway in bladder cancer.