Abstract: OBJECTIVE To investigate the effect of stearic acid grafted chitosan oligosaccharide polymeric micelles loading podophyllotoxin(CSO-SA/PPT) on the cell proliferation in human glioma cells. METHODS CSO-SA/PPT were prepared to investigate its physical and chemical properties. U87 cell proliferative activity was measured by tumor cell uptake assay and MTT assay. RESULTS The CSO-SA/PPT had greater inhibition on human glioma cells proliferation compared with free podophyllotoxin formulation. And in vitro drug uptake tests showed that CSO-SA/PPT presented more uptake characteristics than free podophyllotoxin. CONCLUSION CSO-SA/PPT plays an inhibitory role in the cell proliferation in human glioma cells.