Abstract: OBJECTIVE To establish a method for quality evaluation and validate its feasibilities by simultaneous quantitative assay of four phenylethanoid glycosides in Orobanche cernua Loefling by multi-components with single marker. METHODS Four main phenylethanoid glycosides were selected to evaluate the quality of Orobanche cernua Loefling. Taking acteoside as internal reference substance, and the relative correlation factors of crenatoside, 2'-acetylacteoside and isocrenatoside were determined. Their contents in 24 batches of Orobanche cernua Loefling were determined by both external standard method and quantitative analysis multi-components by single marker(QAMS). RESULTS No significant differences were found in the quantitative results in 24 batches of Orobanche cernua Loefling determined by external standard method and QAMS. CONCLUSION It is feasible and suitable to evaluate the quality of Orobanche cernua Loefling by QAMS.