| 引用本文: | 胡筱希,陆国寿,黄周锋,黄建猷,谭晓,甘吉丽.瑶药翼核果中翼核果素和翼核果醌-Ⅰ的含量测定及抗氧化活性研究[J].中国现代应用药学,2019,36(7):820-823. |
| HU Xiaoxi,LU Guoshou,HUANG Zhoufeng,HUANG Jianyou,TAN Xiao,GAN Jili.Determination and Evaluation on Anti-oxidant Activity of Ventilagolin and Ventiloquinone-Ⅰ in Ventilago Leiocarpa Benth[J].Chin J Mod Appl Pharm(中国现代应用药学),2019,36(7):820-823. |
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| 瑶药翼核果中翼核果素和翼核果醌-Ⅰ的含量测定及抗氧化活性研究 |
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胡筱希1, 陆国寿1,2, 黄周锋1, 黄建猷1, 谭晓1, 甘吉丽3
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1.广西中医药研究院, 南宁 530022;2.广西中药质量标准研究重点实验室, 南宁 530022;3.广西中医药大学药学院, 南宁 530022
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| 摘要: |
| 目的 建立翼核果中的翼核果素及翼核果醌-Ⅰ的含量测定方法，对翼核果素及翼核果醌-Ⅰ抗氧化活性进行初步评价。方法 采用HPLC对翼核果药材中的翼核果素及翼核果醌-Ⅰ的含量进行测定，并进行方法学考察；通过测定翼核果素及翼核果醌-Ⅰ对DPPH自由基的清除能力，以半数清除浓度（IC50）值作为评价清除DPPH自由基能力的指标。结果 翼核果药材中翼核果素及翼核果醌-Ⅰ的含量分别为0.56%，0.10%。2种成分清除DPPH自由基的IC50值分别为1.13，1.63 mg·mL-1。结论 2种成分的定量分析方法分离度良好，重复性好，抗氧化方法简便、快捷，为翼核果的深入研究提供了实验依据。 |
| 关键词: 翼核果素 翼核果醌-Ⅰ 含量测定 抗氧化活性 |
| DOI：10.13748/j.cnki.issn1007-7693.2019.07.010 |
| 分类号:R917.101 |
| 基金项目:广西自然科学基金项目（2016GXNSFAA380092）；广西中药质量标准研究重点实验室自主研究课题（桂中重自201506，桂中重自201601） |
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| Determination and Evaluation on Anti-oxidant Activity of Ventilagolin and Ventiloquinone-Ⅰ in Ventilago Leiocarpa Benth |
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HU Xiaoxi1, LU Guoshou1,2, HUANG Zhoufeng1, HUANG Jianyou1, TAN Xiao1, GAN Jili3
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1.Guangxi Institute of Traditional Medical and Pharmaceutical Sciences, Nanning 530022, China;2.Guangxi Key Laboratory of Traditional Chinese Medicine Quality Stardands, Nanning 530022, China;3.Guangxi University of Chinese Medicine, College of Pharmacy, Nanning 530022, China
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| Abstract: |
| OBJECTIVE To establish a method for simultaneous determination of ventilagolin and ventiloquinone-Ⅰ from Ventilago leiocarpa Benth and to evaluate DPPH free radical scavenging for the anti-oxidant activity. METHODS HPLC method was used to determine the content of ventilagolin and ventiloquinone-Ⅰ and methodologically inspected. Taking IC50 as the evaluating criterion of scavenging capability by determining the DPPH free radical scavenging rate of ventilagolin and ventiloquinone-Ⅰ. RESULTS The contents of ventilagolin and ventiloquinone-Ⅰ were 0.56% and 0.10%. The DPPH free radical scavenging capability of IC50 were 1.13, 1.63 mg·mL-1. CONCLUSION The method has good separation and good reproducibility. The method is convenient and rapid, as well as provide an experimental to intense research of Ventilago leiocarpa Benth. |
| Key words: ventilagolin ventiloquinone-Ⅰ determination anti-oxidant activity |
