| 引用本文: | 杨志军,邓毅,曼琼,杨秀娟,杨延泽.甘草内生菌代谢物的抗炎作用[J].中国现代应用药学,2018,35(5):633-637. |
| YANG Zhijun,DENG Yi,MAN Qiong,YANG Xiujuan,YANG Yanze.Anti-inflammatory Effect in the Metabolites of Endophytes from Glycyrrhiza Uralensis[J].Chin J Mod Appl Pharm(中国现代应用药学),2018,35(5):633-637. |
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| 摘要: |
| 目的 研究甘草内生菌代谢物的体内外抗炎作用。方法 96孔板中接种巨噬细胞RAW264.7培养，模型组加入1 mg·L-1脂多糖（LPS），各受试药物组分别加入10，20，40 mg·L-1甘草水提液浸膏或内生菌发酵物，2 h后再加入LPS1 mg·L-1培养，检测NO、IL-1β、PGE2的含量。Wistar大鼠90只，随机分为9组，空白组、模型组、阳性对照组给予生理盐水0.01 mL·g-1，甘草水煎液组给予甘草水煎液0.95 g·kg-1，各内生菌代谢物组给予甘草内生菌发酵物0.95 g·kg-1，每日1次，连续灌胃给药5 d。末次给药后1 h，除空白组外，各组大鼠尾静脉注射1 mg·kg-1体质量LPS，制备急性肺炎模型，空白组注射等量生理盐水，阳性对照组按5 mg·kg-1体质量腹腔注射地塞米松给药。6 h后大鼠股动脉取血，检测外周血白细胞计数及血清中TNF-α、IL-6、IL-10的含量。结果 与LPS模型组比较，40 mg·L-1时，甘草浸膏组、5组内生菌代谢物组（JTZB006、JTZB018、JTZB059、JTZB060、JTZB063）巨噬细胞RAW264.7分泌的NO、PGE2、IL-1β的含量显著降低（P<0.05或P<0.01）；与同一质量浓度（40 mg·L-1）的甘草浸膏组比较，JTZB006、JTZB060组巨噬细胞RAW264.7分泌的PGE2的含量显著降低（P<0.05）。与模型组比较，阳性对照组、甘草水煎液组、3组内生菌代谢物组（JTZB006、JTZB060、JTZB063）大鼠外周血中白细胞数量及血清中TNF-α、IL-6的含量均显著降低，IL-10的含量显著升高（P<0.05或P<0.01）；与甘草水煎液组比较，JTZB006、JTZB060、JTZB063组IL-10的含量显著升高（P<0.05或P<0.01）。结论 3株甘草内生菌代谢物（JTZB006、JTZB060、JTZB063）对LPS诱导的炎症模型具有体外及体内抗炎作用。 |
| 关键词: 甘草 内生菌代谢物 抗炎 RAW264.7 大鼠 |
| DOI：10.13748/j.cnki.issn1007-7693.2018.05.003 |
| 分类号:R965.2 |
| 基金项目:国家自然科学基金项目（81360633） |
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| Anti-inflammatory Effect in the Metabolites of Endophytes from Glycyrrhiza Uralensis |
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YANG Zhijun, DENG Yi, MAN Qiong, YANG Xiujuan, YANG Yanze
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Gansu University of Traditional Chinese Medicine, Lanzhou 730000, China
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| Abstract: |
| OBJECTIVE To study anti-inflammatory effect of the metabolites of endophytes isolated from Glycyrrhiza uralensis in vivo and in vitro.METHODS RAW264.7 cells were inoculated onto a 96-well plate. The model group was added with 1 mg·L-1 LPS for culture, and each test drug group was added with Glycyrrhiza water extracts or the fermentation products of endophytes (10, 20, 40 mg·L-1) for 2 h of treatment, then LPS (1 mg·L-1) was added for stimulation. The content of nitric oxide (NO), Interleukin-1β (IL-1β) and Prostaglandin E2 (PGE2) were measured. Ninety Wistar rats were randomly divided into 9 groups, and each group was given the corresponding drug by gavage administration once per day for 5 d. Except the blank group, the other groups were intravenously injected with LPS (1 mg·kg-1) to establish an acute pneumonia model, while the blank group was injected with the same volume of normal saline. After 6 h, blood was collected from rat femoral artery to detect peripheral white blood cell counts and the contents of serum TNF-α, IL-6 and IL-10.RESULTS Compared with LPS model group, when the mass concentration was maintained at 40 mg·L-1, the contents of NO, PGE2 and IL-1β were significantly reduced in extractum Glycyrrhiza group and endophytes fermentation products groups(JTZB006, JTZB018, JTZB059, JTZB060, JTZB063)(P<0.05 or P<0.01). The contents of PGE2 in JTZB006, JTZB060 were significantly lower than those of extractum Glycyrrhiza group at 40 mg·L-1. Compared with model group, the white blood cell counts and the contents of TNF-α and IL-6 were significantly reduced in positive control group, Glycyrrhiza water decoction group, 3 groups of the fermentation products of endophytes. The contents of IL-10 in JTZB006, JTZB060 and JTZB063 significantly higher than those of Glycyrrhiza water decoction group in rat serum (P<0.05 or P<0.01).CONCLUSION JTZB006, JTZB060 and JTZB063 have anti-inflammatory effects in LPS induced inflammatory model. |
| Key words: Glycyrrhiza uralensis the metabolites of endophytes anti-inflammatory RAW264.7 rats |
