| 引用本文: | 莫丽,方晴霞,余陈欢,张欢欢,严洁萍,吕良忠.基于miRNAs的知母水提物体外抑制胃癌细胞SGC7901增殖作用机制[J].中国现代应用药学,2018,35(5):678-683. |
| MO Li,FANG Qingxia,YU Chenhuan,ZHANG Huanhuan,YAN Jieping,LYU Liangzhong.Aqueous Extract from Anemarrhenae Rhizoma Inhibit the Proliferation of SGC7901 Cells via Regulating miRNAs in Vitro[J].Chin J Mod Appl Pharm(中国现代应用药学),2018,35(5):678-683. |
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| 基于miRNAs的知母水提物体外抑制胃癌细胞SGC7901增殖作用机制 |
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莫丽1, 方晴霞2, 余陈欢3, 张欢欢3, 严洁萍2, 吕良忠1,2
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1.浙江中医药大学药学院, 杭州 310053;2.浙江省人民医院药学部, 杭州 310014;3.浙江省医学科学院, 浙江省实验动物与安全性研究重点实验室, 杭州 310013
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| 摘要: |
| 目的 从微小核苷酸（miRNAs）的角度研究知母水提物（aqueous extract from Anemarrhenae Rhizoma，ARAE）体外抑制SGC7901细胞增殖的作用机制。方法 采用MTT法检测ARAE对SGC7901细胞增殖的影响；采用流式细胞术检测ARAE对SGC7901细胞凋亡的影响；采用高通量测序法检测细胞中miRNAs的表达丰度差异；采用miranda、mirbase和targetscan 3个数据库信息比对，预测差异miRNAs的靶基因，并通过KEGG分析靶基因的相关功能；采用实时荧光定量PCR法验证主要靶基因的表达变化。结果 ARAE能明显抑制SGC7901细胞的增殖并诱导其凋亡，调节细胞miR-16-5p、miR-20a-5p、miR-26b-5p和miR-15b-5p的表达水平；并提示这些miRNAs所调控的靶基因主要富集于PI3K-Akt、JAK-STAT和MAPK等信号通路。结论 ARAE可能通过调节SGC7901细胞内miRNAs的表达从而抑制SGC7901细胞增殖，并诱导其凋亡。 |
| 关键词: 知母水提物 SGC7901 微小核苷酸 靶基因 |
| DOI：10.13748/j.cnki.issn1007-7693.2018.05.013 |
| 分类号:R285.5 |
| 基金项目:浙江省科技计划项目（2014F10033，2015F50065） |
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| Aqueous Extract from Anemarrhenae Rhizoma Inhibit the Proliferation of SGC7901 Cells via Regulating miRNAs in Vitro |
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MO Li1, FANG Qingxia2, YU Chenhuan3, ZHANG Huanhuan3, YAN Jieping2, LYU Liangzhong1,2
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1.Pharmacy College, Zhejiang Chinese Medical University, Hangzhou 310053, China;2.Department of Pharmacy, Zhejiang Provincial People's Hospital, Hangzhou 310014 China;3.Zhejiang Key Laboratory of Experimental Animal and Safety Evaluation, Zhejiang Academy of Medical Sciences, Hangzhou 310013, China
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| Abstract: |
| OBJECTIVE To investigate the effects of aqueous extract from Anemarrhenae Rhizoma (ARAE) on the proliferation of SGC7901 in vitro based on miRNA regulation.METHODS The inhibition of ARAE on SGC7901 cell proliferation was evaluated by MTT method. And flow cytometry was used to detect the apoptosis of SGC7901 after ARAE treatment. And after ARAE treatment, the different expressions of miRNAs were detected by high-throughput sequencing analysis. Then target genes of differentially expressed miRNAs were analyzed by using miranda, mirbase and targetscan database, while the target functions were considered by KEGG analysis. Those targets were identified by qRT-PCR.RESULTS ARAE could significantly inhibit the proliferation of SGC7901 cells and induced the apoptosis of SGC7901 as well as regulate the expression of miRNAs including miR-16-5p, miR-20a-5p, miR-26b-5p and miR-15b-5p, which were involved in the signaling regulation of PI3K-Akt, JAK-STAT and MAPK pathways.CONCLUSION ARAE could inhibit the proliferation of SGC7901 and induced cell apoptosis in vitro via regulating the expression of miRNAs. |
| Key words: aqueous extract from Anemarrhenae Rhizoma (ARAE) SGC7901 miRNAs target genes |
