| 引用本文: | 向秋玲,李雪兰.蓬子菜总黄酮对四氯化碳诱导的小鼠肝损伤的保护作用及机制[J].中国现代应用药学,2017,34(10):1392-1396. |
| XIANG Qiuling,LI Xuelan.Effect and Mechanism of Total Flavonoids from Galium Verum L. on CCl4-induced Liver Injury in Mice[J].Chin J Mod Appl Pharm(中国现代应用药学),2017,34(10):1392-1396. |
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| 摘要: |
| 目的 探讨蓬子菜总黄酮（total flavonoids extracts of Galium verum L.，TFG）对CCl4致小鼠急性肝损伤的影响及其机制。方法 60只昆明小鼠随机分成正常组、模型组、阳性对照组、TFG组。各治疗组予以相应的药物灌胃6 d后，除正常组外其余各组腹腔注射6% CCl4造模，24 h后取样，肝组织HE染色，检测血清中谷草转氨酶（AST）、谷丙转氨酶（ALT）以及肝肾组织中超氧化物歧化酶（SOD）、谷胱甘肽（GSH）、丙二醛（MDA）水平，分析血清中TNF-α和IL-6水平。昆明小鼠50只，随机分成正常组、模型组、TFG组（200 mg·kg-1）、抗IL-6单抗（40 mg·kg-1）+TFG（200 mg·kg-1）组、抗TNF-α单抗（5 mg·kg-1）+TFG（200 mg·kg-1）组，各治疗组予以相应的药物处理6 d后，除正常组外，其余各组腹腔注射6% CCl4造模，24 h后取血清分析AST、ALT水平。结果 与模型组比较，蓬子菜总黄酮能改善肝损伤，降低血清中AST、ALT活性以及肝肾中MDA含量，提高肝肾SOD活性、GSH含量，降低血清中TNF-α和IL-6表达（P<0.05或P<0.01）。抗IL-6、TNF-α单抗能明显降低血清中AST、ALT活性（P<0.05）。结论 蓬子菜总黄酮能通过清除自由基、抑制脂质过氧化，保护细胞膜和线粒体膜的完整性。同时减少IL-6、TNF-α的释放，改善急性肝损伤。 |
| 关键词: 蓬子菜 总黄酮 急性肝损伤 氧化应激 抗炎 |
| DOI：10.13748/j.cnki.issn1007-7693.2017.10.007 |
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| Effect and Mechanism of Total Flavonoids from Galium Verum L. on CCl4-induced Liver Injury in Mice |
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XIANG Qiuling, LI Xuelan
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Department of Medicine of Changde Vocational Technical College, Changde 415000, China
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| Abstract: |
| OBJECTIVE To explore the effect and mechanism of total flavonoids extracts of Galium verum L.(TFG) on CCl4-induced liver injury in mice. METHODS Sixty KM mice were randomly divided into the normal group, the model group, the positive control group and TFG group. The mice in each treatment group were given corresponding drugs for 6 d. Except the normal group, the other groups were injected intraperitoneally with 6% CCl4 for 24 h, HE staining was used. Activitise of serum aspartate aminotransferase(AST), alanine aminotransferase(ALT) and levels of albumin superoxide dismutase(SOD), glutathione(GSH-Px), and malondialdehyde (MDA) in liver and kidney tissue were detected, levels of serum TNF-α and IL-6 were analyzed. Fifty KM mice were randomly divided into normal group, model group, TFG group(200 mg·kg-1), anti-TNF-α monoclonal antibody(40 mg·kg-1)+TFG(200 mg·kg-1) group, anti-IL-6 monoclonal antibody(5 mg·kg-1)+TFG(200 mg·kg-1) group. The mice in each treatment group were given corresponding drugs for 6 d. Except the normal group, the other groups were injected intraperitoneally with 6% CCl4 for 24 h. Finally, activitise of serum AST and ALT were measured. RESULTS Compared with the model group, TFG could improve CCl4-induced liver injury in mice, significantly decrease activitise of serum AST, ALT and level of MDA in liver and kidney, increase activities of SOD and content of GSH in liver and kidney, significantly decreased the levels of serum TNF-α and IL-6(P<0.05 or P<0.01). Anti-IL-6 and TNF-α monoclonal antibody could significantly decrease activities of serum AST and ALT(P<0.05). CONCLUSION TFG can effectively improve the CCl4-induced liver injury in mice, which may be related to its ability to improve antioxidant, inhibit lipid peroxidation, protect the integrity of the cell membrane and mitochondrial membrane, while reducing the release of IL-6 and TNF-α. |
| Key words: Galium verum L. total flavonoid liver injury oxidative stress anti-inflammatory |
