HPLC测定当归中1-(3',4'-dihydroxycinnamoyl)-cyclopentane-2,3-diol的含量

罗镭; 马临科; 刘帅英; 祝明<sup>*</sup>

引用本文:	罗镭,马临科,刘帅英,祝明.HPLC测定当归中1-(3',4'-dihydroxycinnamoyl)-cyclopentane-2,3-diol的含量[J].中国现代应用药学,2017,34(9):1296-1299.
	LUO Lei,MA Linke,LIU Shuaiying,ZHU Ming.Determination of the 1-(3',4'-Dihydroxycinnamoyl)-cyclopentane-2,3-diol in Angelicae Sinensis Radix by HPLC[J].Chin J Mod Appl Pharm(中国现代应用药学),2017,34(9):1296-1299.

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HPLC测定当归中1-(3',4'-dihydroxycinnamoyl)-cyclopentane-2,3-diol的含量
罗镭¹, 马临科¹, 刘帅英², 祝明¹
1.浙江省食品药品检验研究院, 杭州 310052;2.丽水市食品药品与质量技术检验检测院, 浙江丽水 323000

摘要:

目的建立HPLC测定当归中1-（3'，4'-dihydroxycinnamoyl）-cyclopentane-2，3-diol含量的方法。方法采用Inertsil ODS-3 C₁₈柱（4.6 mm×250 mm，5 μm），流动相为乙腈-0.05%三氟乙酸水溶液梯度洗脱，流速为1.0 mL·min^-1，检测波长为325 nm，柱温为30℃。结果 1-（3'，4'-dihydroxycinnamoyl）-cyclopentane-2，3-diol进样量在0.023 36~1.168 0 μg（r=1.000 0）内与峰面积呈良好的线性关系，平均回收率为95.33%，RSD为1.88%。结论该方法简便、准确、重复性好，可为当归药材的质量控制提供参考。

关键词: 当归高效液相色谱法 1-(3',4'-dihydroxycinnamoyl)-cyclopentane-2,3-diol 含量测定

DOI：10.13748/j.cnki.issn1007-7693.2017.09.017

分类号:R917.101

基金项目:浙江省中医药科技计划（2017ZA097）

Determination of the 1-(3',4'-Dihydroxycinnamoyl)-cyclopentane-2,3-diol in Angelicae Sinensis Radix by HPLC

LUO Lei¹, MA Linke¹, LIU Shuaiying², ZHU Ming¹

1.Zhejiang Institute for Food and Drug Control, Hangzhou 310052, China;2.Lishui Inspection Institute for Food and Drug & Quality and Technology Control, Lishui 323000, China

Abstract:

OBJECTIVE To establish an HPLC method for determination of 1-(3',4'-dihydroxycinnamoyl)-cyclopentane -2,3-diol in Angelicae Sinensis Radix. METHODS Inertsil ODS-3 C₁₈ column (4.6 mm×250 mm, 5 μm) was adopted with a mobile phase of acetonitrile-0.05% trifluoroacetic acid at the flow rate of 1.0 mL·min^-1. The detection wavelength was set at 325 nm and the column temperature was 30℃. RESULTS The good linearity with peak areas was achieved in the rang of 0.023 36-1.168 0 μg (r=1.000 0) for 1-(3',4'-dihydroxycinnamoyl)-cyclopentane-2,3-diol. The average recovery was 95.33%, and the RSD was 1.88%. CONCLUSION The method is simple, accurate and reproducible, and can be used for the quality control of Angelicae Sinensis Radix.

Key words: Angelicae Sinensis Radix HPLC 1-(3',4'-dihydroxycinnamoyl)-cyclopentane-2,3-diol content determination