| 引用本文: | 余立雁,潘荣华,王倩倩,郑立忠,马忠俊.LC-TOF/MS检测功能性红曲中非法添加异源洛伐他汀的研究[J].中国现代应用药学,2017,34(2):266-268. |
| YU Liyan,PAN Ronghua,WANG Qianqian,ZHENG Lizhong,MA Zhongjun.Adulteration Detection of Lovastatin in Red Yeast Rice by LC-TOF/MS[J].Chin J Mod Appl Pharm(中国现代应用药学),2017,34(2):266-268. |
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| 摘要: |
| 目的 建立基于LC-TOF/MS技术的分析方法，检测功能性红曲中非法添加非红曲来源的洛伐他汀。方法 红曲样品的甲醇提取物经Zorbax SB-C18（4.6 mm×150 mm，5 μm）色谱柱，以0.05%甲酸水溶液-乙腈为流动相梯度洗脱（0~40min，40%~100%乙腈），正离子扫描检测。结果 非法添加非红曲来源的洛伐他汀的功能性红曲存在天然功能性红曲没有的特征杂质离子峰，可以区分非法添加非红曲来源的洛伐他汀的功能性红曲和天然功能性红曲。结论 本方法可用于天然功能性红曲是否非法添加非红曲来源的洛伐他汀的鉴别。 |
| 关键词: 红曲 非法添加 洛伐他汀 杂质 |
| DOI：10.13748/j.cnki.issn1007-7693.2017.02.024 |
| 分类号:R284.1；R917.101 |
| 基金项目: |
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| Adulteration Detection of Lovastatin in Red Yeast Rice by LC-TOF/MS |
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YU Liyan1, PAN Ronghua2, WANG Qianqian1, ZHENG Lizhong2, MA Zhongjun1
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1.Ocean College, Zhejiang University, Zhoushan 316021, China;2.Zhejiang Sanhe Bio-tech Co., Ltd., Jiangshan 324123, China
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| Abstract: |
| OBJECTIVE To establish an analysis method to detect the adulteration of lovastatin in red yeast rice by LC-TOF/MS.METHODS Methanol extract of red yeast rice was subject to LC-MS analysis by applying Zorbax SB-C18 (4.6 mm×150 mm, 5 μm) column, eluted using water supplied with 0.05% formic acid-acetonitrile with gradient elucidation (0~40 min, 40%~100% acetonitrile), and scanned in positive ion mode.RESULTS Comparison analysis led to the finding of typical ions could specifically distinguish adulterated lovastatin in red yeast rice.CONCLUSION The established LC-MS analysis method can be applied for adulteration detection of lovastatin in red yeast rice. |
| Key words: red yeast rice adulteration lovastatin impurities |
