| 引用本文: | 李志峰,邓漫漫,查洁,周勇,方志鸿,徐兵.Apatinib对急性髓系白血病干祖细胞样细胞株kg1α的杀伤作用及其分子机制[J].中国现代应用药学,2017,34(2):204-209. |
| LI Zhifeng,DENG Manman,ZHA Jie,ZHOU Yong,FANG Zhihong,XU Bing.Apatinib Inhibits Proliferation and Induces Apoptosis of Acute Myeloid Leukemia Stem/progenitor Like Cell Line (kg1α cells) and Its Mechanism[J].Chin J Mod Appl Pharm(中国现代应用药学),2017,34(2):204-209. |
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| 摘要: |
| 目的 研究新型小分子酪氨酸激酶抑制剂Apatinib对急性髓系白血病（acute myeloid leukemia，AML）干祖细胞增殖和凋亡的影响及其相关分子机制。方法 CCK8法检测不同浓度Apatinib对kg1α细胞的增殖抑制作用，Annexin V/PI法检测不同浓度Apatinib诱导kg1α细胞和原代CD34+AML干细胞的凋亡情况，Western blot法检测Apatinib处理kg1α细胞后PI3K/AKT通路相关蛋白（AKT、Raf和PTEN）的表达变化。结果 不同浓度的Apatinib （2.5，5，10，20，40μmol·L-1）作用48 h和72 h后对kg1α细胞均具有显著的增殖抑制作用，呈浓度和时间依赖性，与对照组相比差异均具有统计学意义（P<0.05或P<0.01）。Annexin V/PI检测细胞凋亡的结果显示，不同浓度apatinib对kg1α具有显著的诱导凋亡作用，作用48 h和72 h后的凋亡率和对照组相比差异均有统计学意义（P<0.01）。不同浓度Apatinib作用48 h后对7例原代AML干细胞均具有显著的杀伤作用，与对照组相比差异具有显著的统计学意义（P<0.01）；Western blot结果显示，Apatinib处理kg1α细胞48 h后AKT/p-AKT、p-Raf表达降低，而p-PTEN表达增加。结论 Apatinib可抑制AML干祖细胞样细胞kg1α细胞的增殖，且可诱导kg1α细胞和原代CD34+AML干祖细胞的凋亡，均呈浓度和时间依赖性，其作用机制可能是通过干扰PI3K/AKT通路实现的。 |
| 关键词: Apatinib AML干祖细胞样细胞株(kg1α) PI3K/AKT pathway |
| DOI：10.13748/j.cnki.issn1007-7693.2017.02.012 |
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| Apatinib Inhibits Proliferation and Induces Apoptosis of Acute Myeloid Leukemia Stem/progenitor Like Cell Line (kg1α cells) and Its Mechanism |
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LI Zhifeng, DENG Manman, ZHA Jie, ZHOU Yong, FANG Zhihong, XU Bing
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Department of Hematology, The First Affiliated Hospital of Xiamen University, Xiamen 361003, China
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| Abstract: |
| OBJECTIVE To investigate the effect of Apatinib, a small-molecule vascular endothelial growth factor receptor-2 tyrosine kinase inhibitor, on the proliferation and apoptosis of acute myeloid leukemia(AML) stem/progenitor cells and its molecule mechanism.METHODS The kg1α cells and primary CD34+ AML stem cells were treated with a serial of concentrations of Apatinib for 48 h and 72 h, the inhibitory ratio was measured by CCK8 assay, the apoptosis percent was measured by flow cytometry. Western bolt was used to analyzed AKT/p-AKT, p-Raf and p-PTEN expression after treatment with 0, 10, 20 μmol·L-1 Apatinib in kg1α cells.RESULTS After treatment with a serial of Apatinib (2.5, 5, 10, 20, 40 μmol·L-1) on AML stem-like cell line(kg1α) for 48 h and 72 h, the cell proliferation were significantly inhibited in a dose-and time-dependent mode. All the differences had statistical significance compared with control group. The results of Annexin V/PI showed that various concentration of Apatinib induced significantly apoptosis on kg1α cells. After 48 h and 72 h, all the apoptosis percentage were significantly higher than control group(P<0.01); Apatinib significantly induced apoptosis of 7 cases primary AML stem cell, the difference has statistical significance; Western blot results indicated decrease the expression of AKT/p-AKT and p-Raf, however, upregulated the level of p-PTEN.CONCLUSION Apatinib can inhibit the proliferation of AML stem/progenitor like cell line(kg1α cells) and also induce the apoptosis of kg1α and primary CD34+ AML stem cells. Its mechanism may be related with the PI3K/AKT signal pathway. |
| Key words: Apatinib acute myeloid leukemia stem/progenitor like cell (kg1α) PI3K/AKT pathway |
