| 引用本文: | 李丽，李勇文，段小群，陈旭，杨新平，梁成钦，卢曦，曾建红.罗汉果甜苷V特异性抗血清的制备与应用[J].中国现代应用药学,2012,29(7):632-634. |
| LI Li, LI Yongwen, DUAN Xiaoqun, CHEN Xu, YANG Xingping, LIANG Chengqin, LU Xi, ZENG Jianhong.Preparation of Mogrosides V-specific Antiserum and Its Application[J].Chin J Mod Appl Pharm(中国现代应用药学),2012,29(7):632-634. |
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| 摘要: |
| 目的 制备罗汉果甜苷V(MogV)特异性抗血清，建立MogV含量竞争性抑制酶联免疫检测法。方法 用罗汉果甜苷V-丁二酸酯-牛血清蛋白(MogV-HS-BSA)结合比约为44∶1为免疫抗原制备抗血清，用罗汉果甜苷V-丁二酸酯-人血清蛋白(MogV-HS-HSA)结合比约为64∶1为包被抗原，建立MogV含量竞争性抑制酶联免疫检测法。结果 包被抗原工作浓度为1 μg·mL-1，血清最佳稀释倍数为1∶8 000，在此工作浓度下，MogV浓度在1×10-4~1 μg·mL-1内回归方程Y=-0.158X+0.298，r=0.991 0。结论 成功制备了MogV抗血清，MogV 浓度在1×10-4~1 μg·mL-1内对抗血清的OD(405 nm)值抑制作用呈良好的线性关系。 |
| 关键词: 罗汉果甜苷V 特异性抗血清 制备 竞争抑制 |
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| 基金项目:科技部科技人员服务企业项目(2009GJE10040)；广西自然科学基金项目面上项目(2010GXNSFA013251)；广西教育厅面上项目(200911MS170) |
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| Preparation of Mogrosides V-specific Antiserum and Its Application |
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LI Li, LI Yongwen, DUAN Xiaoqun, CHEN Xu, YANG Xingping, LIANG Chengqin, LU Xi, ZENG Jianhong
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Guilin Medical University, Guilin 541004, China
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| Abstract: |
| OBJECTIVE To prepare mogrosides V (MogV) specific antiserum and establish the competitive-inhibitive ELISA method to detect MogV content. METHODS Antiserum was prepared with the V-Mogrosides succinate-bovine serum albumin(MogV-HS-BSA) as the immunogen (ratio about 44∶1), the competitive inhibition ELISA method for MogV content was established with the V-Mogrosides succinate-human serum protein (MogV-HS-HSA) with a ratio of about 64∶1 as coating antigens. RESULTS The coating antigen working concentration was 1 μg·mL-1, the best serum dilution is 1∶8 000. Within MogV concentration 1×10-4-1 μg·mL-1, the regression equation was Y=-0.158X+0.298, r=0.991 0. CONCLUSION MogV anti-serum is successfully prepared. Within 1×10-4-1 μg·mL-1, the MogV concentration and OD value(405 nm) of the antiserum shows a good linear relationship. |
| Key words: mogrosides V specific antiserum preparation competitive inhibition |
