| 引用本文: | 刘群，黄欣，荆俊丽，苏乐群.不同方法提取的元胡白芷产物中相关药效成分及镇痛作用的比较[J].中国现代应用药学,2012,29(5):415-418. |
| LIU Qun, HUANG Xin, JING Junli, SU Lequn.Comparison of Products from Corydalis Rhizama and Angelica Dahuricae Radix with Different Extraction Methods on Active Components and Analgesic Effects[J].Chin J Mod Appl Pharm(中国现代应用药学),2012,29(5):415-418. |
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| 摘要: |
| 目的 对二氧化碳超临界萃取(CO2-SFE)和醇回流方法所提取的元胡白芷两种不同产物进行比较。方法 采用固含物量、薄层色谱(TLC)、高效液相(HPLC)和不同的大鼠疼痛模型，从相关药效成分、提取物镇痛等角度对两种提取方法所得的产物进行比较。结果 CO2-SFE法所得萃取物的量约为醇回流法的1/4，纯度为后者的6～9倍；CO2-SFE法的提取物的镇痛作用强于醇回流法，且对血中疼痛物质的影响优于醇回流法。结论 在元胡白芷的提取上，CO2-SFE明显优于醇回流技术，适宜进一步推广应用。 |
| 关键词: 元胡 白芷 二氧化碳超临界萃取 醇回流提取 |
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| 基金项目:山东省科技攻关计划项目(2007GG2NS02053) |
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| Comparison of Products from Corydalis Rhizama and Angelica Dahuricae Radix with Different Extraction Methods on Active Components and Analgesic Effects |
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LIU Qun, HUANG Xin, JING Junli, SU Lequn1,2
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1.School of Pharmaceutical Sciences of Shandong University, Ji’nan 250012, China;2.Pharmaceutical Department, Shandong Province Qianfoshan Hospital, Ji’nan 250014, China
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| Abstract: |
| OBJECTIVE To compare the two different extracting methods:the CO2-SFE and the alcohol refluxing method, on extraction of Corydalis Rhizama and Angelica dahuricae Radix. METHODS TLC, HPLC were used to determine the extracting efficiency with the active components yield; the analgesic experiments were used to explore the differences in the analgesic effect between the two extraction methods. RESULTS CO2-SFE had 1/4 extract yield of refluxing method, but its purity was 6-9 times higher than refluxing method. It had better analgesic effect in the experimental models and had better effects on pain-related substances. CONCLUSION To extract Corydalis Rhizama and Angelica dahuricae Radix by SFE is more efficient and feasible than the traditional alcohol refluxing method. |
| Key words: Corydalis Rhizama Angelica dahuricae Radix CO2-SFE alcohol refluxing method |
