| 引用本文: | 刘斌,王涛,张明,丁富娟.桔贝合剂HPLC特征图谱的建立和HPLC-ELSD同时测定6种成分含量[J].中国现代应用药学,2023,40(13):1827-1832. |
| LIU Bin,WANG Tao,ZHANG Ming,DING Fujuan.Establishment of Characteristic HPLC Chromatograms and Simultaneous Determination of Six Components by HPLC-ELSD of Jubei Mixture[J].Chin J Mod Appl Pharm(中国现代应用药学),2023,40(13):1827-1832. |
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| 桔贝合剂HPLC特征图谱的建立和HPLC-ELSD同时测定6种成分含量 |
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刘斌, 王涛, 张明, 丁富娟
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泰安市食品药品检验检测研究院, 山东 泰安 271000
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| 摘要: |
| 目的 建立桔贝合剂的HPLC特征图谱,并采用HPLC-ELSD同时测定其中6种成分的含量。方法 建立桔贝合剂HPLC特征图谱,色谱柱为Agilent ZORBAX-C18(250 mm×4.6 mm,5 μm),流动相为乙腈(A)-0.1%磷酸水溶液(B),梯度洗脱,流速为1.0 mL·min-1,柱温为30℃,检测波长为250 nm;含量测定采用HPLC-ELSD,色谱柱为Waters T3 C18(250 mm×4.6 mm,5 μm),流动相为乙腈(A)-1%乙酸水溶液(B),梯度洗脱,流速为1.0 mL·min-1,柱温为30℃。结果 建立了以黄芩苷为参照峰的HPLC特征图谱,确定了8个共有特征峰;苦杏仁苷、甘草苷、贝母素甲、贝母素乙、桔梗皂苷D和麦冬皂苷D在各自范围内线性关系良好(r ≥ 0.999),平均回收率分别为99.1%,98.6%,100.9%,97.7%,98.9%和98.0%,RSD分别为0.4%,1.0%,1.1%,1.2%,0.7%,1.3%。结论 本研究建立的方法可对桔贝合剂进行定性鉴别和定量测定,能够有效评价与控制桔贝合剂质量。 |
| 关键词: 桔贝合剂 HPLC特征图谱 苦杏仁苷 甘草苷 贝母素甲 贝母素乙 桔梗皂苷D 麦冬皂苷D |
| DOI：10.13748/j.cnki.issn1007-7693.20221510 |
| 分类号:R284.1 |
| 基金项目: |
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| Establishment of Characteristic HPLC Chromatograms and Simultaneous Determination of Six Components by HPLC-ELSD of Jubei Mixture |
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LIU Bin, WANG Tao, ZHANG Ming, DING Fujuan
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Tai'an Testing Institute for Food and Drug Control, Tai'an. 271000, China
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| Abstract: |
| OBJECTIVE To establish characteristic HPLC chromatograms for Jubei mixture, and to simultaneous determine its six components by HPLC-ELSD. METHODS An HPLC specific chromatogram of the Jubei mixture was established. The chromatographic column was Agilent ZORBAX-C18(250 mm×4.6 mm, 5 μm), gradient elution was conducted with acetonitrile(A)-0.1% phosphoric acid aqueous solution(B) as the mobile phase, the flow rate was 1.0 mL·min-1, and the column temperature was 30℃, the detection wavelength was 250 nm. An HPLC-ELSD method was established and the chromatography was accomplished on a Waters T3 C18(250 mm×4.6 mm, 5 μm) column, the mobile phase consisted of acetonitrile(A)-1% acetic acid aqueous solution(B) in a gradient mode, the flow rate was 1.0 mL·min-1, the column temperature was set at 30℃. RESULTS The characteristic chromatograms of the HPLC with baicalin as the reference peak was established, and eight common peaks were defined. The linear ranges of amygdalin, liquiritin, peimine, peiminine, platycodin D and ophiopogonin D were good within their own ranges(r ≥ 0.999). The average recovery were 99.1%, 98.6%, 100.9%, 97.7%, 98.9% and 98.0%, RSD were 0.4%, 1.0%, 1.1%, 1.2%, 0.7% and 1.3%, respectively. CONCLUSION The established methods can be used to qualitative identification and quantitative determination of Jubei mixture, can effectively used for evaluation and quality control of Jubei mixture. |
| Key words: Jubei mixture HPLC specific chromatogram amygdalin liquiritin peimine peiminine platycodin D ophiopogonin D |
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