| 引用本文: | 李俊1，赵海明2，常方方1，李全民3.罗红霉素的水杨基荧光酮荷移分光光度法测定[J].中国现代应用药学,2009,(8):664-667. |
| LI Jun1, ZHAO Haiming2, CHANG Fangfang1, LI Quanmin3.Determination of Roxithromycin by Spectrophotometry Based on Charge-transfer Reaction with Salicyl Fluorone[J].Chin J Mod Appl Pharm(中国现代应用药学),2009,(8):664-667. |
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| 摘要: |
| 目的测定罗红霉素胶囊中罗红霉素的含量，增加质量控制和保证方法。方法利用罗红霉素与水杨基荧光酮(SAF)形成1∶1荷移络合物，应用分光光度法测定胶囊中罗红霉素含量。结果络合物的最大吸收波长是516 nm，表观摩尔吸光系数ε为5.01×103 L·mol-1·cm-1，罗红霉素在33.5～217.6 mg·L-1内符合比尔定律，RSD为0.64%(n=8)，平均回收率为100.9%。结论方法简单、灵敏、准确，可作为该药的含量测定方法。 |
| 关键词: 罗红霉素 水杨基荧光酮 荷移反应 分光光度法 |
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| Determination of Roxithromycin by Spectrophotometry Based on Charge-transfer Reaction with Salicyl Fluorone |
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LI Jun1, ZHAO Haiming2, CHANG Fangfang1, LI Quanmin3
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| Abstract: |
| OBJECTIVE To determine roxithromycin in capsules and provide the quality control and assurance. METHODS A spectrophotometric method based on the charge-transfer reaction between salicyl fluorone (SAF) and roxithromycin. RESULTS The molar absorptivity of the complex at 516 nm was 5.01×103 L·mol-1·cm-1. Beer's law was obeyed in the range of 33.5-217.6 mg L-1of roxithromycin. The relative standard deviation was 0.64%(n=8). The composition of the complex was found to be 1∶1 by slope ratio and Job’s methods. The mechanism of reaction was investigated, and the average recovery was found to be 100.9%. CONCLUSION The method is simple, quick and accurate for the determination of roxithromycin in capsules. |
| Key words: roxithromycin salicyl fluorone charge-transfer reaction spectrophotometry |
