Simultaneous Determination of Chlorogenic Acid, Caffeic Acid and Vanillic Acid in Sargentodoxae Caulis by HPLC

OBJECTIVE To develop an HPLC method for simultaneous determination of chlorogenic acid, caffeic acid and vanillic acid in each fraction of Sargentodoxae Caulis. METHODS The analysis was carried on a column of Kromasil C₁₈(4.6 mm×250 mm, 4.5 μm). Isocratic elution, mobile phase consisted of solvent A (1%, solution of HAc in water) and solvent B (methanol) at the ratio of 68∶32, the flow rate was 1.0 mL.min^-1, the injection volume was 20 μL, the detection wavelength was 327 nm, the column temperature was at 30 ℃. RESULTS Ethyl acetate fraction had the highest concentration of chlorogenic acid (4.52%) and caffeic acid (0.11%) and dichloromethane fraction possessed the highest concentration of vanillic acid of 5.8%. CONCLUSION This method is simple, accurate, rapid and suitable for the usual quality control of Sargentodoxae Caulis.