Determination and Purification Technology of Total Phenols from Picris Japonica Thunb.

OBJECTIVE To establish a method for the content determination and purification technology of total phenols from Picris japonica Thunb. METHODS The content of total phenols was determined by UV-visible spectrophotometry and the purification technology of total phenols by macroporous resin was optimized with extent of adsorption, adsorption rate, desorption amount and desorption rate as index. RESULTS The RSD of precision, reproducibility and stability of the quality control method were <3.0%. HPD-20 macroporous resin showed good purified capability to total phenols from Picris japonica Thunb.. Optimum technology conditions were determined as follow: 1 BV of sample solution (the content of total phenols was 8.54 mg·mL^-1) was passed through microporous resin with elution rate of 2 BV·h^-1, after eluted with 4 BV of water and 4 BV of 10% ethanol, then collected 4 BV of 30% ethanol eluent and 3 BV of 50% ethanol eluent, and concentrated the combined eluent under vacuum to yield the purified total phenols. CONCLUSION The method established for determined the content of total phenols from Picris japonica Thunb. is convenient and accurate. After used HP-20 resin and the optimized technology, the purity of total phenols from Picris japonica Thunb. is >87%, and the process is stable.