Protect Effects of Huperzine A on Methylglyoxal Induced Injury in the Cultured Human Brain Microvascular Endothelial Cell in Vitro Experimental Study

OBJECTIVE To evaluate the effects of huperzine A on the methylglyoxal (MGO) induced injury in the cultured human brain microvascular endothelial cell (HBMEC). METHODS HBMEC cell line was chosen to induce MGO injury. Cell vitality was measured by using MTT, LDH release, SOD activity were tested by kits. Cell apoptosis was measured by caspase 3 activity. RESULTS Huperzine A dose-dependently protected MGO induced HBMEC injury. At 10^-5 mol?L^-1 of huperzine A manifested the maximum effects. MGO increased SOD activity, which were reversed by pretreatment of venlafaxine (10^-6 and 10^-5 mol?L^-1). Furthermore, huperzine A (10^-6 and 10^-5 mol?L^-1) also decreased MGO induced caspase 3 activity increasing. CONCLUSION Huperzine A protected MGO induced injury in the cultured HBMEC, which may be involved its anti-oxidation and anti-apoptosis activity.