Simultaneous Determination of Seven Active Compounds in Bailing Anshen Tablets by UPLC

OBJECTIVE To established a UPLC method for determination of seven active compounds in Bailing Anshen tablets, including chlorogenic acid, mangiferin, tetrahydroxystilbene, spinosin, cynaroside, quercitrin, and 3,5-dicaffeoylquinic. METHODS The chromatographic separation performed on a C18 column and with gradient elution of acetonitrile and 0.1% aqueous phosphoric acid at a flow rate of 0.4 mL·min^-1. The detected wavelength was set at 340 nm. RESULTS The seven analytes showed good linearity (r>0.999 8) within the test ranges. The average recoveries were 96.3%, 98.2%, 100.5%, 99.3 %, 97.7%, 97.0%, 96.4%, respectively. CONCLUSION The analytical method is simple, rapid, specific, which is useful for quality control of Bailing Anshen tablets.