Study on Deproteinization Methods of Polysaccharide from Metaplexis Japonica Nutshell

OBJECTIVE To elect the best deproteinization method of polysaccharide from Metaplexis Japonica nutshell. METHODS The protein was removed by the methods of Sevag, TCA, papain-sevag and papain-TCA. The rate of deproteinization and the loss rate of polysaccharide were detected. RESULTS The percentage of deproteinization of Sevag, TCA, papain-Sevag and papain-TCA were 73.14%, 71.55%, 86.03%, 75.33% respectively and the loss rate of polysaccharide were 14.40%, 9.22%, 8.47%, 7.13% respectively. CONCLUSION The best deproteinization method of polysaccharide from Metaplexis Japonica nutshell was the papain-Sevag method.