Toxicity Study of the Uptaking Compositions of Diosooroa Bulbifera L Alcohol Extract on HL-7702 and HepG2 Cell

OBJECTIVE To study the toxicity of the uptaking compositions of Diosooroa bulbifera L alcohol extract on HL-7702 and HepG2 cell. METHODS After reflux extraction with 75% alcohol, the alcohol extracts of Diosooroa bulbifera L at high, middle and low doses were added to Caco-2 cell model. Then the uptaking compositions were added to HL-7702 and HepG2 cell for the determination of the cytoactive and the biochemical indicator of ALT, AST, GSH-PX and MDA. RESULTS Compared with the control group, the high and middle dose groups decreased the survival rate of HL-7702 and HepG2 cell (P<0.01). The high dose group increased ALT and AST of HL-7702 cell supernatant after 72 h (P<0.01). The high dose group increased ALT and AST of HepG2 cell supernatant after 48 h and 72 h (P<0.01). MDA were significant increased and GSH-PX were significant decreased in the supernatant of HL-7702 and HepG2 cell at high and middle doses after 48 h and 72 h (P<0.01). CONCLUSION The result showed that the uptaking compositions of Diosooroa bulbifera L alcohol extract had toxic effect in HL-7702 and HepG2 cell.