Analysis of HPLC Characteristic Spectrum on Dendrobium Officinale of Fengdous

OBJECTIVE To establish the method of characteristic spectrum analysis on Dendrobium officinale of Fengdous by HPLC. METHODS HPLC was used with the Zorbax SB C₁₈ column, the acetonitrile-0.2%methanoic acid solution with gradient elution was employed as a mobile phase, detection wavelength was 270 nm, column temperature was 35 ℃, and flow rate was 1.0 mL·min^-1. RESULTS Thirty six common peaks were separated in Dendrobium officinale of Fengdous, the similarities of 10 batches were 0.898-0.969. CONCLUSION The method is accurate and reliable, has a better reproducibility, and provides a reference for the quality control of Dendrobium officinale of Fengdous.