The Penetrating Activity of Nano-silver Mediated by Cell Penetrating Peptide

OBJECTIVE To assess the intracellular delivery efficiency of silver nanoparticles surface-modified by cell penetrating peptides(TAT) in human breast cancer drug doxorubicin-resistant MCF-7/ADR cells. METHODS Silver nanoparticle(AgNP) was prepared by chemical reduction and was modified with TAT by Ag-S covalent bond. The TAT-AgNP was characterized using particle analyzer, TEM, laser confocal microsopy, and TGA, and TAT-mediated cellular uptake were measured using FACS. RESULTS The AgNP with diameter below 10 nm was successfully synthesized. The cell penetrating activity of TAT-modified AgNP (AgNP-TAT) was higher than that of AgNP. CONCLUSION The cell penetrating activity of AgNP was significantly improved by modification with TAT.