Determination of Fluconazole and Moxifloxacin in Situ Forming of Eye Gel by HPLC

OBJECTIVE To establish an HPLC method for measuring the content of fluconazole, moxifloxacin and ethylparaben in situ forming of eye gel. METHODS ODS-C₁₈ column was used, triethylamine buffer-methanol as mobile phase, the flow rate was 1.0 mL·min^-1, detection wavelength was 260 nm. RESULTS The linear range of fluconazole, moxifloxacin and ethylparaben were 25-250 μg·mL^-1(r=0.999 9, n=6), 25-250 μg·mL^-1(r=0.999 9, n=6), 2.5-25 μg·mL^-1 (r=0.999 7, n=6), the average recovery rates were 99.6%, 99.4%, 99.3%. The RSD were 1.23%, 1.20%, 1.17%. CONCLUSION The method is simple, accurate, sensitive and the reproductivity is good. It can be used for the determination of this preparation.