Simultaneously Determination of Chlorogenic Acid and Baicalin in Yinhuang Granules by HPLC

OBJECTIVE To develop an HPLC method for the simultaneously determination of chlorogenic acid and baicalin in Yinhuang granules. METHODS HPLC was carried out, using Agilent Eclipse XDB-C18 (4. 6 mm ×150 mm, 5 μm) column set at 30 ℃, the acetonitrile-0.1% H3PO4 as mobile phase(using gradient) with flowing rate of 1.0 mL·min-1. The detection wavelength was set at 327 nm. RESULTS The method had good linear relationship within the range of 0.021-2.1 μg (r =0.999 8) for chlorogenic acid and 0.038-3.800 μg (r =0.999 6) for baicalin,respectively. The results of the average recovery were 99.70% (RSD=0.75%) and 99.06% (RSD=1.02%). CONCLUSION The method established was simple and accurate and can be used to simultaneously determine chlorogenic acid and baicalin in Yinhuang granules.