Determination of Cefixime by Micellar Electrokinetic Capillary Chromatography

OBJECTIVE To develop a method of micellar electrokinetic capillary chromatography for determination of cefixime. METHODS The uncoated fused-silica capillary (51 cm×75 μm) was used.The running buffer composed of 70 mmol·L-1 phosphate buffer containing 100 mmol·L-1 sodium dodecylsulphate(pH 7.0).The sample was injected to the column by pressure (50 mbar) for 5 s.The separation voltage was 15 kV and the capillary temperature was 25℃.The detection wavelength was set at 254 nm with acetanilide as internal standard. RESULTS The calibration curve was linear within the range of 6.08-777.98 μg·mL-1 for cefixime (r=0.999 3).The limit of detection and limit of quantitation were 1.03 μg·mL-1 and 3.43 μg·mL-1 respectively.Statistical analysis by t-test showed no significant differences between the results obtained by micellar electrokinetic capillary chromatography (MECC) and by high performance liquid chromatography (HPLC). CONCLUSION The MECC method developed was accurate ,simple and sensitive,and could be complement to HPLC.