Optimization of the Extraction Process of Huangshen Electuary by Apoptosis of Human Gastric Cancer Cells in Vitro

OBJECTIVE To optimize the extraction technology for Huangshen Electuary. METHODS The effects of three factors including the times of extraction, time length of extraction and solvent ratio were investigated, and each factor was chosen three levels. Optimize extraction of Huangshen Electuary in the L9(34) orthogonal test, as apoptosis of human gastric cancer cells in vireo as an index. RESULTS The best extraction technology is: adopt 8 times quantities of water to boil for 2 times,1 hours for each time. CONCLUSION The optimum extraction technology for Huangshen Electuary is feasibie. The induction of human gastric cancer cells apoptosis is one of Huangshen Electuary effect mechanism.