Content Determination of the Essential Oil from Lavender with Different Extraction Methods by GC

OBJECTIVE To determine the linalyl acetate and linalool content in the essential oil from lavender with different extraction methods by GC. METHODS The capillary GC system consisted of a DB-5 capillary column(30 m×0.25 mm, 0.25 μm), and meta-dinitro-benzent was used as an internal standard. The detector was FID; inlet temperature was 230 ℃; split ratio was 20∶1; the flow rate was 1.0 mL·min^-1; the detector temperature was 250 ℃. The incept temperature programmed was 110 ℃ and kept for 3 min; programmed from 110 ℃ to 130 ℃ at 10 ℃·min^-1 and kept for 4 min, and then progammed to 250 ℃ at 20 ℃·min^-1 and kept for 1 min. RESULTS Linalool, linalyl acetate and the internal standard were separated in the chromatographic condition. The linalool content from the supercritical CO₂ extract in the volatile oil was 38.02%, the linalyl acetate content was 34.24%, the linalool content from the steam distillation extract in the volatile oil was 59.09%, the linalyl acetate content was 18.14%. CONCLUSION The method is accurate, selectivity, and can be used as quality control for the lavender oil extracted with different methods.