Determination of N(2)-L-alanyl-L-glutamine by Post-Column Derivatization with Ninhydrin

OBJECTIVE To establish a method using post-column derivatization with ninhydrin for determination of N(2)-L-alanyl-L-glutamine(L-Ala-L-Glu). METHODS According to principle of solution having maximum absorption in 570 nm, potassium sodium buffer as mobile phase, L-Ala-L-Glu was eluted and reacted with ninhydrin, the production can be detected by spectrophotometer, amino acid analyzer determined the content of L-Ala-L-Glu. RESULTS The linear relation was good in the range of 1-20 mg·L^-1 (r=0.997 9). The recoveries of low, middle and high concentration were 94.5%, 97.6% and 98.7% with the RSD of 0.52%, 0.10% and 0.06%, respectively. CONCLUSION This method is simple, separating degree is good, results are accurate and reliable, and can be used in the determination of L-Ala-L-Glu.