Determination of Gallic Acid and Quercitrin in Euphorbia Hirta L. by HPLC

OBJECTIVE To establish an HPLC method for the determination of gallic acid and quercitrin in Euphorbia hirta L. METHODS Using ZORBAX SB-Aq(4.6 mm×250 mm, 5 μm)column, at 30 ℃; methanol-acetonitrile-0.2% phosphoric acid solution was used as the mobile phase, with gradient elution. The flow rate was 1.0 mL·min^-1. The detection wavelength was changed by time. RESULTS The gallic acid and quercitrin had a good linearity relationship between 2.328-58.20 mg·mL^-1 and 6.804-170.1 mg·mL^-1, with r=0.999 5 and 0.999 8. The average recovery rate were 100.26% and 96.29%, with RSD of 0.64% and 0.69%(n=9). CONCLUSION The method is simple, rapid and accurate, suitable for the quality control of Euphorbia hirta L.